Individual cells capable of interferon-γ/(IFN-γ) synthesis are easily detected by immunofluorescence and flow cytometric analysis using an anti- IFN-γ monoclonal antibody as specific reagent. By IFN-γ flow cytometry assay, we demonstrated that HIV-seropositive patients, starting at the early stage of viral infection, generally have an increased percentage of lymphocytes potentially able to produce IFN-γ, compared with healthy blood donors, IFN-γ expression in patient lymphocytes was observed to increase with the progressive stages of HIV infection, with the highest figures occurring in stage C patients. Such increased IFN-γ expression involved both CD4+ and CD8+ T cell subsets. Most interestingly, we found patients at the same stage of HIV infection who had similar numbers of total and CD4+ lymphocytes but highly different percentages of lymphocytes potentially capable of producing IFN-γ.
Titolo: | CD4+ and CD8+ Lymphocytes of Patients with AIDS Synthesize Increased Amounts of Interferon-gamma. |
Autori: | |
Data di pubblicazione: | 1995 |
Rivista: | |
Abstract: | Individual cells capable of interferon-γ/(IFN-γ) synthesis are easily detected by immunofluorescence and flow cytometric analysis using an anti- IFN-γ monoclonal antibody as specific reagent. By IFN-γ flow cytometry assay, we demonstrated that HIV-seropositive patients, starting at the early stage of viral infection, generally have an increased percentage of lymphocytes potentially able to produce IFN-γ, compared with healthy blood donors, IFN-γ expression in patient lymphocytes was observed to increase with the progressive stages of HIV infection, with the highest figures occurring in stage C patients. Such increased IFN-γ expression involved both CD4+ and CD8+ T cell subsets. Most interestingly, we found patients at the same stage of HIV infection who had similar numbers of total and CD4+ lymphocytes but highly different percentages of lymphocytes potentially capable of producing IFN-γ. |
Handle: | http://hdl.handle.net/11379/8187 |
Appare nelle tipologie: | 1.1 Articolo in rivista |