The sulfatide fluorescent analogue N-l&amine rhodaminyl-(12-aminododecanoyl)cerehroside 3-sulfate was admin- istered in the form of albumin complex to normal human skin tibroblasts and its metabolic fate was investigated. Ceramide, galactosylceramide, glucosylceramide, sphingomyelin and free acid, all containing the fluorophore lissamine rhodamine, have been synthesized as reference standards for the identification of the metabolic products. Ceramide appeared to be the main metabolic product present both in cell extract and medium, followed by galactosylceramide and sphingomyelin. Fluorescence microscopy of cells showed a marked perinuclear fluorescence.
Uptake and metabolism of a fluorescent sulfatide analogue in cultured skin fibroblasts
MONTI, Eugenio;PRETI, Augusto;ALEO, Maria Francesca;MARCHESINI, Sergio
1992-01-01
Abstract
The sulfatide fluorescent analogue N-l&amine rhodaminyl-(12-aminododecanoyl)cerehroside 3-sulfate was admin- istered in the form of albumin complex to normal human skin tibroblasts and its metabolic fate was investigated. Ceramide, galactosylceramide, glucosylceramide, sphingomyelin and free acid, all containing the fluorophore lissamine rhodamine, have been synthesized as reference standards for the identification of the metabolic products. Ceramide appeared to be the main metabolic product present both in cell extract and medium, followed by galactosylceramide and sphingomyelin. Fluorescence microscopy of cells showed a marked perinuclear fluorescence.File | Dimensione | Formato | |
---|---|---|---|
monti1992.pdf
solo utenti autorizzati
Descrizione: Articolo principale
Tipologia:
Full Text
Licenza:
NON PUBBLICO - Accesso privato/ristretto
Dimensione
901.76 kB
Formato
Adobe PDF
|
901.76 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.