Skin tissue injuries require careful and specialized management due to the complexity of the healing processes involved. Recent advancements in regenerative medicine are centered around the development of innovative scaffolds that can function as delivery systems to restore the lost tissue functions and properties. This study investigates the fabrication and characterization of two hydrogels: a gelatin-polyethylene glycol-chitosan-glycerol-based hydrogel (G-PEG-CH-Gly) and a gelatin-polyethylene glycol-chitosan-based hydrogel (G-PEG-CH). The biological properties of these scaffolds were assessed in terms of biocompatibility with human mesenchymal stromal cells (hMSCs) derived from bone marrow (BM-hMSCs) and umbilical cord (UC-hMSCs). MatriDerm (MD) was used as a positive control for biological evaluation. BM/UC-hMSCs were differentiated into fibroblastic and keratinocyte lineages in the presence of fetal bovine serum (FBS) or human platelet lysate (HPL), both in two-dimensional (2D) culture and three-dimensional (3D) scaffolds. The results indicated that G-PEG-CH-Gly and G-PEG-CH were more effective than MD in inducing keratinocyte and fibroblastic differentiation, highlighting their potential role in promoting cell differentiation. Furthermore, UC-hMSCs exhibited higher expression levels of fibroblast and keratinocyte markers compared to BM-hMSCs, with the best results achieved with G-PEG-CH over G-PEG-CH-Gly and MD. This study suggests that novel CH-based hydrogels could represent a promising therapeutic strategy for skin wound healing.
Design and Characterization of a 3D In Vitro Model of Chitosan/Glycerol-Based Hydrogels With Human Mesenchymal Stromal Cells for Skin Wound Healing
Re F.;Sartore L.;Borsani E.;Bosio K.;Trenta F.;Giugno L.;Bianchetti A.;Russo D.
2025-01-01
Abstract
Skin tissue injuries require careful and specialized management due to the complexity of the healing processes involved. Recent advancements in regenerative medicine are centered around the development of innovative scaffolds that can function as delivery systems to restore the lost tissue functions and properties. This study investigates the fabrication and characterization of two hydrogels: a gelatin-polyethylene glycol-chitosan-glycerol-based hydrogel (G-PEG-CH-Gly) and a gelatin-polyethylene glycol-chitosan-based hydrogel (G-PEG-CH). The biological properties of these scaffolds were assessed in terms of biocompatibility with human mesenchymal stromal cells (hMSCs) derived from bone marrow (BM-hMSCs) and umbilical cord (UC-hMSCs). MatriDerm (MD) was used as a positive control for biological evaluation. BM/UC-hMSCs were differentiated into fibroblastic and keratinocyte lineages in the presence of fetal bovine serum (FBS) or human platelet lysate (HPL), both in two-dimensional (2D) culture and three-dimensional (3D) scaffolds. The results indicated that G-PEG-CH-Gly and G-PEG-CH were more effective than MD in inducing keratinocyte and fibroblastic differentiation, highlighting their potential role in promoting cell differentiation. Furthermore, UC-hMSCs exhibited higher expression levels of fibroblast and keratinocyte markers compared to BM-hMSCs, with the best results achieved with G-PEG-CH over G-PEG-CH-Gly and MD. This study suggests that novel CH-based hydrogels could represent a promising therapeutic strategy for skin wound healing.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
