beta-Galactosylceramidase (GALC) is a lysosomal enzyme involved in sphingolipid metabolism by removing beta-galactosyl moieties from beta-galactosyl ceramide and beta-galactosyl sphingosine. Previous observations have shown that GALC exerts a pro-oncogenic activity in human melanoma. Here, the impact of GALC overexpression on the proteomic landscape of BRAF-mutated A2058 and A375 human melanoma cell lines was investigated by liquid chromatography-tandem mass spectrometry analysis of the cell extracts. The results indicate that GALC overexpression causes the upregulation/downregulation of 172/99 proteins in GALC-transduced cells when compared to control cells. Gene ontology categorization of up/down-regulated proteins indicates that GALC may modulate the protein landscape in BRAF-mutated melanoma cells by affecting various biological processes, including RNA metabolism, cell organelle fate, and intracellular redox status. Overall, these data provide further insights into the pro-oncogenic functions of the sphingolipid metabolizing enzyme GALC in human melanoma.Dataset: The data set has been submitted as a supplement to this paper.Dataset License: license under which the dataset is made available (CC0, CC-BY, CC-BY-SA, CC-BY-NC, etc.)
Dataset: Impact of β-Galactosylceramidase Overexpression on the Protein Profile of Braf(V600E) Mutated Melanoma Cells
Capoferri D.;Chiodelli P.;Calza S.;Presta M.
2023-01-01
Abstract
beta-Galactosylceramidase (GALC) is a lysosomal enzyme involved in sphingolipid metabolism by removing beta-galactosyl moieties from beta-galactosyl ceramide and beta-galactosyl sphingosine. Previous observations have shown that GALC exerts a pro-oncogenic activity in human melanoma. Here, the impact of GALC overexpression on the proteomic landscape of BRAF-mutated A2058 and A375 human melanoma cell lines was investigated by liquid chromatography-tandem mass spectrometry analysis of the cell extracts. The results indicate that GALC overexpression causes the upregulation/downregulation of 172/99 proteins in GALC-transduced cells when compared to control cells. Gene ontology categorization of up/down-regulated proteins indicates that GALC may modulate the protein landscape in BRAF-mutated melanoma cells by affecting various biological processes, including RNA metabolism, cell organelle fate, and intracellular redox status. Overall, these data provide further insights into the pro-oncogenic functions of the sphingolipid metabolizing enzyme GALC in human melanoma.Dataset: The data set has been submitted as a supplement to this paper.Dataset License: license under which the dataset is made available (CC0, CC-BY, CC-BY-SA, CC-BY-NC, etc.)I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
