Objectives: Carbapenems are one of the last-report therapeutic choices to treat infections due to multidrug-resistant (MDR) micro-organisms. For this reason, the spread of carbapenemase-producing Enterobacteriaceae represents a serious health-public problem. Here we describe isolates co-producing bla(NDM-5 )and bla(OXA-1).Methods: Three Escherichia coli isolates obtained from patients with invasive infections were analysed by phenotypic antibiotic susceptibility testing and whole-genome sequencing (WGS).Results: All of the isolates were resistant to carbapenems, most beta-lactam antibiotics, piperacillin/tazobactam, amoxicillin/clavulanic acid and ciprofloxacin, remaining susceptible to amikacin, fosfomycin, colistin and tigecycline. The isolates belonged to sequence types ST44, ST405 and ST167 and co-harboured the bla(NDM-5 )and bla(OXA-1) genes. Two of the isolates also harboured extended-spectrum beta-lactamase (ESBL) genes (bla(CTX-M-15 )and bla(TEM-1b)). The bla(NDM-5) gene was probably carried chromosomally even if different plasmids were identified. Various virulence genes were also identified.Conclusion: Our results highlight that continuous surveillance is essential to monitor the spread of clinically important MDR pathogens. (C) 2022 The Authors. Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.
Genomic characterisation of Escherichia coli isolates co-producing NDM-5 and OXA-1 from hospitalised patients with invasive infections
Corbellini, S;Gurrieri, F;Mascherpa, M;Boroni, G;Amolini, C;De Francesco, M A
2022-01-01
Abstract
Objectives: Carbapenems are one of the last-report therapeutic choices to treat infections due to multidrug-resistant (MDR) micro-organisms. For this reason, the spread of carbapenemase-producing Enterobacteriaceae represents a serious health-public problem. Here we describe isolates co-producing bla(NDM-5 )and bla(OXA-1).Methods: Three Escherichia coli isolates obtained from patients with invasive infections were analysed by phenotypic antibiotic susceptibility testing and whole-genome sequencing (WGS).Results: All of the isolates were resistant to carbapenems, most beta-lactam antibiotics, piperacillin/tazobactam, amoxicillin/clavulanic acid and ciprofloxacin, remaining susceptible to amikacin, fosfomycin, colistin and tigecycline. The isolates belonged to sequence types ST44, ST405 and ST167 and co-harboured the bla(NDM-5 )and bla(OXA-1) genes. Two of the isolates also harboured extended-spectrum beta-lactamase (ESBL) genes (bla(CTX-M-15 )and bla(TEM-1b)). The bla(NDM-5) gene was probably carried chromosomally even if different plasmids were identified. Various virulence genes were also identified.Conclusion: Our results highlight that continuous surveillance is essential to monitor the spread of clinically important MDR pathogens. (C) 2022 The Authors. Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
