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Nerve growth factor-endothelial cell interaction leads to angiogenesis in vitro and in vivo.

PRESTA, Marco;
2002-01-01
2002
MIUR (compresi PRIN FIRB,FISR)
LS4_8 Non-communicable diseases (except for neural/psychiatric, immunity-related, metabolism-related disorders, cancer and cardiovascular diseases)
FASEB JOURNAL
2-s2.0-0036674499
WOS:000176683900020
Inglese
16
1307
1309
2
12154004
Nerve growth factor (NGF) has important functions during embryonic development and on various tissues and organs under normal and pathological conditions during the extrauterine life. RT-PCR analysis and immunological methods demonstrate that human umbilical vein endothelial cells (HUVECs) express the NGF receptors trkA(NGFR) and p75NTR. NGF treatment caused a rapid phosphorylation of trkA(NGFR) in HUVECs, determining a parallel increase of phosphorylated ERK1/2. Accordingly, NGF induced a significant increase in HUVEC proliferation that was abolished by the trkA(NGFR) inhibitor K252a. Also, HUVECs express significant levels of NGF under standard culture conditions that were up-regulated during serum starvation. Endogenous NGF was responsible for the basal levels of trkA(NGFR) and ERK1/2 phosphorylation observed in untreated HUVEC cultures. Finally, NGF exerted a potent, direct, angiogenic activity in vivo when delivered onto the chorioallantoic membrane of the chicken embryo. The data indicate that NGF may play an important role in blood vessel formation in the nervous system and in several pathological processes, including tumors and inflammatory diseases. Unraveling mechanisms of NGF-dependent angiogenesis could provide valuable tools for novel therapeutic approaches in antiangiogenic therapy.
Animals; Autocrine Communication; Blood Vessels; Cell Division; Cells; Cultured; Chick Embryo; Endothelium; Vascular; Extraembryonic Membranes; Humans; Models; Biological; Neovascularization; Physiologic; Nerve Growth Factor; RNA; Messenger; Receptor; trkA
Sogg. privati ital. no profit
http://dx.doi.org/10.1096/fj.01-1000fje
9
info:eu-repo/semantics/article
262
G., Cantarella; L., Lempereur; Presta, Marco; D., Ribatti; G., Lombardo; P., Lazarovici; G., Zappalà; C., Pafumi; R., Bernardini
1 Contributo su Rivista::1.1 Articolo in rivista
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1.1 Articolo in rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11379/31964
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