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The zebrafish/tumor xenograft angiogenesis assay.

NICOLI, Stefania;PRESTA, Marco
2007-01-01
2007
MIUR (compresi PRIN FIRB,FISR)
LS4_8 Non-communicable diseases (except for neural/psychiatric, immunity-related, metabolism-related disorders, cancer and cardiovascular diseases)
NATURE PROTOCOLS
2-s2.0-39149110311
WOS:000253140000031
Inglese
2
2918
2923
5
18007628
Here we describe a method to study tumor angiogenesis in zebrafish (Danio rerio) based on the injection of proangiogenic mammalian tumor cells into the perivitelline space of zebrafish embryos at 48 h post-fertilization. Within 24-48 h, proangiogenic tumor grafts induce a neovascular response originating from the developing subintestinal vessels. This can be observed at macroscopic and microscopic levels after whole-mount alkaline phosphatase staining of wild-type zebrafish embryos, or by fluorescence microscopy in transgenic VEGFR2:G-RCFP embryos in which endothelial cells express the green fluorescent protein under the control of the VEGFR2/KDR promoter. Angiogenesis inhibitors added to the injected cell suspension or to the fish water prevent tumor-induced neovascularization. The assay is rapid and inexpensive, representing a novel tool for investigating tumor angiogenesis and for antiangiogenic drug discovery. Also, gene inactivation by antisense morpholino oligonucleotides injection in zebrafish embryos may allow the identification of genes involved in tumor angiogenesis.
Animals; Genetically Modified; Disease Models; Animal; Embryo; Nonmammalian; Green Fluorescent Proteins; Neoplasms; Neovascularization; Pathologic; Tumor Cells; Cultured; Xenograft Model Antitumor Assays; Zebrafish
Sogg. privati ital. no profit
http://dx.doi.org/10.1038/nprot.2007.412
2
info:eu-repo/semantics/article
262
Nicoli, Stefania; Presta, Marco
1 Contributo su Rivista::1.1 Articolo in rivista
none
1.1 Articolo in rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11379/31956
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