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Trop-2 overexpression as an independent marker for poor overall survival in ovarian carcinoma patients.

BIGNOTTI, Eliana;TODESCHINI, Paola;CALZA, Stefano;RAVANINI, Maria;TASSI, Renata Alessandra;RAVAGGI, Antonella;BANDIERA, Elisabetta;ROMANI, Chiara;ZANOTTI, Laura;ODICINO, Franco;FACCHETTI, Fabio;PECORELLI, Sergio;SANTIN, Alessandro
2010-01-01
2010
Enti pubb. di ricerca ital.
LS4_8 Non-communicable diseases (except for neural/psychiatric, immunity-related, metabolism-related disorders, cancer and cardiovascular diseases)
EUROPEAN JOURNAL OF CANCER
2-s2.0-77349099069
WOS:000276755800023
Sì, ma tipo non specificato
Inglese
Internazionale
-
-
20060709
BACKGROUND: Prognostic factors currently available are insufficient to predict the clinical course of epithelial ovarian cancer (EOC). In a previous microarray study we identified the human trophoblast cell surface antigen Trop-2 as one of the top differentially expressed genes in serous papillary EOCs compared to normal human ovarian surface epithelial (HOSE) short-term cultures. The aim of the present investigation was to analyse Trop-2 expression at mRNA and protein level and to assess its prognostic significance in EOC. METHODS: Using quantitative real-time PCR we tested a total of 104 fresh-frozen EOC tissues and 24 HOSE for Trop-2 mRNA expression. Trop-2 protein expression was then examined by immunohistochemistry in matched formalin-fixed paraffin-embedded EOC samples and in 13 normal ovaries. Finally, we correlated Trop-2 expression to EOC conventional clinicopathological features and patient outcomes. RESULTS: We found a significant Trop-2 mRNA and protein upregulation in EOCs compared to normal controls (p<0.001). Trop-2 protein overexpression was significantly associated with the presence of ascites (p=0.04) and lymph node metastases (p=0.04). By univariate survival analysis, Trop-2 protein overexpression was significantly associated with decreased progression-free (p=0.02) and overall survival (p=0.01). Importantly, Trop-2 protein overexpression was an independent prognostic marker for shortened survival time in multivariate Cox regression analysis (p=0.04, HR=2.35, CI(95\%)=1.03-5.34). CONCLUSIONS: Our results indicate, for the first time, that Trop-2 protein overexpression correlates with an aggressive malignant phenotype and may constitute a novel prognostic factor for EOC. The targeting of Trop-2 overexpression by immunotherapeutic strategies may represent an attractive and potentially effective approach in patients harbouring EOC.
http://www.ncbi.nlm.nih.gov/pubmed/20060709
15
info:eu-repo/semantics/article
262
Bignotti, Eliana; Todeschini, Paola; Calza, Stefano; M., Falchetti; Ravanini, Maria; Tassi, Renata Alessandra; Ravaggi, Antonella; Bandiera, Elisabett...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11379/30239
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