The major histopathological finding in the Alzheimer’s disease (AD) brain is the presence of neuritic plaques containing β-amyloid (Aβ). In addition some findings showed an alteration of nitric oxide pathway in AD patients. PDGF-hAPP mice, an animal model of AD, deposit Aβ in age-dependent manner, were used as. In this study, we investigated the neuronal nitric oxide synthase (nNOS) expression in the cortex of transgenic PDGF-hAPP mice comparing with C57BL/6 control. We also detected neu-ropeptide Y (NPY), known to be co-localised with NOS in cortical neurons, and neuN, a neuronal marker. Our results showed a significant decrease in the number of nNOS positive neurons in the cortex of transgenic mice respect to control. NADPH-d/NPY double staining showed that almost all nitroxidergic neurons were co-localized with NPY and that several neurons were only NPY positive. Respect to control, transgenic mice showed a decrease of NADPH-d/NPY double stained neurons and an apparent increase of NPY single stained neurons. The total number of neuN positive neurons was similar in both groups, suggesting that in some double stained neurons there was a nNOS down-regulation but that the neurons survived expressed only NPY. These data support that nitroxidergic system is impaired in this AD animal model.
Expression of neuronal nitric oxide synthase in PDGF-hAPP transgenic mice
BORSANI, Elisa;STACCHIOTTI, Alessandra;REZZANI, Rita;RODELLA, Luigi Fabrizio
2006-01-01
Abstract
The major histopathological finding in the Alzheimer’s disease (AD) brain is the presence of neuritic plaques containing β-amyloid (Aβ). In addition some findings showed an alteration of nitric oxide pathway in AD patients. PDGF-hAPP mice, an animal model of AD, deposit Aβ in age-dependent manner, were used as. In this study, we investigated the neuronal nitric oxide synthase (nNOS) expression in the cortex of transgenic PDGF-hAPP mice comparing with C57BL/6 control. We also detected neu-ropeptide Y (NPY), known to be co-localised with NOS in cortical neurons, and neuN, a neuronal marker. Our results showed a significant decrease in the number of nNOS positive neurons in the cortex of transgenic mice respect to control. NADPH-d/NPY double staining showed that almost all nitroxidergic neurons were co-localized with NPY and that several neurons were only NPY positive. Respect to control, transgenic mice showed a decrease of NADPH-d/NPY double stained neurons and an apparent increase of NPY single stained neurons. The total number of neuN positive neurons was similar in both groups, suggesting that in some double stained neurons there was a nNOS down-regulation but that the neurons survived expressed only NPY. These data support that nitroxidergic system is impaired in this AD animal model.File | Dimensione | Formato | |
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Ricci F - v3 p605 Med Hypoth Res 2006.pdf
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