Atrial fibrillation becomes a self-perpetuating arrhythmia as a consequence of electrophysiogic and structural remodeling involving the atrium. Oxidative stress may be a link between this rhythm disturbance and electrophysiogic remodeling. The aim of this study was to evaluate whether the heme oxygenase-1 (HO-1) marker of oxidative stress was more expressed in left atrial sites with stronger structural remodeling in patients affected by chronic atrial fibrillation (CAF) and mitral valve disease (MD). Myocardial samples were taken from the left atrial posterior wall (LAPW) and left atrial appendage (LAA) of 24 patients with CAF-MD in addition to 10 autopsy controls. The levels of HO-1 messenger RNA (mRNA) and HO-1 protein in each pathologic LAPW and LAA were quantified using reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay. Furthermore, light microscopy was used to morphometrically evaluate the differential myocyte and interstitial changes in the same CAF-MD LAPWand LAA samples. In controls, HO-1 protein was quantified using enzyme-linked immunosorbent assay. Unlike controls, patients with CAF-MD had higher levels of HO-1 mRNA and its protein product, expressed as LAPW/LAA ratios, in the LAPW (2.18 ± 1.18, P<.0001, and 1.55 ± 0.67, P b .005), and their LAPW also showed greater histologic changes in myocytolytic myocytes (15.1% ± 3.1% versus 6.9% ± 3.3%, P<.0001), interstitial fibrosis (8.2% ± 2.2% versus 2.8% ± 1.2%, P<.0001), and capillary density (816 ± 120 number/mm2 versus 1114 ± 188 number/mm2; P<.05). In addition, markers of oxidative stress were immunohistochemically studied with antinitrotyrosine and anti-iNOS antibodies. In patients with CAF-MD, the inducible enzyme HO-1 is more expressed in the left atrial areas that show greater structural remodeling. This finding strongly suggests a pathogenetic relationship between oxidative stress and the degree of histologic change. © 2008 Elsevier Inc. All rights reserved.
Heme oxygenase-1 expression in the left atrial myocardium of patients with chronic atrial fibrillation related to mitral valve disease: its regional relationship with structural remodeling
BENUSSI S;DE PALMA, Giuseppe;
2008-01-01
Abstract
Atrial fibrillation becomes a self-perpetuating arrhythmia as a consequence of electrophysiogic and structural remodeling involving the atrium. Oxidative stress may be a link between this rhythm disturbance and electrophysiogic remodeling. The aim of this study was to evaluate whether the heme oxygenase-1 (HO-1) marker of oxidative stress was more expressed in left atrial sites with stronger structural remodeling in patients affected by chronic atrial fibrillation (CAF) and mitral valve disease (MD). Myocardial samples were taken from the left atrial posterior wall (LAPW) and left atrial appendage (LAA) of 24 patients with CAF-MD in addition to 10 autopsy controls. The levels of HO-1 messenger RNA (mRNA) and HO-1 protein in each pathologic LAPW and LAA were quantified using reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay. Furthermore, light microscopy was used to morphometrically evaluate the differential myocyte and interstitial changes in the same CAF-MD LAPWand LAA samples. In controls, HO-1 protein was quantified using enzyme-linked immunosorbent assay. Unlike controls, patients with CAF-MD had higher levels of HO-1 mRNA and its protein product, expressed as LAPW/LAA ratios, in the LAPW (2.18 ± 1.18, P<.0001, and 1.55 ± 0.67, P b .005), and their LAPW also showed greater histologic changes in myocytolytic myocytes (15.1% ± 3.1% versus 6.9% ± 3.3%, P<.0001), interstitial fibrosis (8.2% ± 2.2% versus 2.8% ± 1.2%, P<.0001), and capillary density (816 ± 120 number/mm2 versus 1114 ± 188 number/mm2; P<.05). In addition, markers of oxidative stress were immunohistochemically studied with antinitrotyrosine and anti-iNOS antibodies. In patients with CAF-MD, the inducible enzyme HO-1 is more expressed in the left atrial areas that show greater structural remodeling. This finding strongly suggests a pathogenetic relationship between oxidative stress and the degree of histologic change. © 2008 Elsevier Inc. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
