A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was validated and used to quantify bcr/abl b3a2 and b2a2 mRNAs in clinical samples. bcr/abl mRNA was correlated to Philadelphia chromosome levels and differed significantly between cytogenetic response groups. A bcr/abl mRNA threshold value correctly identified 96% of samples without a major or complete cytogenetic response.
Quantification of bcr/abl mRNA expression by a rapid real-time reverse transcription-polymerase chain reaction assay in patients with chronic myeloid leukemia.
ZANELLA, Isabella;FINAZZI, Dario;ALBERTINI, Alberto;
2001-01-01
Abstract
A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was validated and used to quantify bcr/abl b3a2 and b2a2 mRNAs in clinical samples. bcr/abl mRNA was correlated to Philadelphia chromosome levels and differed significantly between cytogenetic response groups. A bcr/abl mRNA threshold value correctly identified 96% of samples without a major or complete cytogenetic response.File in questo prodotto:
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2001 Haematologica.pdf
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