OBJECTIVE: Two single nucleotide polymorphisms at position 680 and 307 of the FSH receptor gene have been associated with infertility and ovar- ian response in IVF. The aim of this study was to examine the frequency dis- tribution of FSH receptor polymorphisms in infertile patients and controls and to correlate these polymorphisms with the outcome of IVF cycle. DESIGN: Case-control study. MATERIALS AND METHODS: 86 IVF infertile patients undergoing ovarian stimulation in a long suppression protocol and 60 fertile controls were included in the study. Fertile patients included were enrolled among women who delivered and had no history of infertility. Blood samples were obtained and DNA was analyzed to determine the FSH receptor geno- type. Among IVF cycle: basal FSH levels, number of antral follicles, number of preovulatory follicles (>15mm), number of retrieved oocytes, duration of stimulation (days), number of transfer embryo, pregnancy rate and amount of FSH were assessed and correlated with genotypes. RESULTS: No statistically significant differences in the distribution of polymorphisms were found among the group of infertile women and controls. Basal FSH levels, number of antral follicles, number of preovulatory follicles (>15mm), number of retrieved oocytes and number of transfered embryos was comparable between infertile patients with different FSH receptor geno- type. However, patients who carry TT (thr-thr) and NN (asn-asn) genotype need fewer days of ovarian stimulation to oocyte retrieval. (TT: 9,07 days, p1⁄40,028; NN: 9,08 days, p1⁄40,037). A trend of better pregnancy rate in the AA genotype carrying patients was found, although not statistically signifi- cant. The dose of FSH required for a successful stimulation was similar in the two polymorphisms. CONCLUSIONS: FSH receptor genotype is not different between infertile and fertile patients. TT and NN genotype acchieve a significantly higher ovarian response. There is a not significant trend for better pregnancy rate in AA subgroup patients. Our results seem to support some role of FSH re- ceptor polymorphism in infertile patients: the clinical relevance of this factor is however probably low.
FSH receptor polymorphisms in a population of infertile women and controls
OMODEI, Umberto;MARCHINA, Eleonora;BARLATI, Sergio;
2008-01-01
Abstract
OBJECTIVE: Two single nucleotide polymorphisms at position 680 and 307 of the FSH receptor gene have been associated with infertility and ovar- ian response in IVF. The aim of this study was to examine the frequency dis- tribution of FSH receptor polymorphisms in infertile patients and controls and to correlate these polymorphisms with the outcome of IVF cycle. DESIGN: Case-control study. MATERIALS AND METHODS: 86 IVF infertile patients undergoing ovarian stimulation in a long suppression protocol and 60 fertile controls were included in the study. Fertile patients included were enrolled among women who delivered and had no history of infertility. Blood samples were obtained and DNA was analyzed to determine the FSH receptor geno- type. Among IVF cycle: basal FSH levels, number of antral follicles, number of preovulatory follicles (>15mm), number of retrieved oocytes, duration of stimulation (days), number of transfer embryo, pregnancy rate and amount of FSH were assessed and correlated with genotypes. RESULTS: No statistically significant differences in the distribution of polymorphisms were found among the group of infertile women and controls. Basal FSH levels, number of antral follicles, number of preovulatory follicles (>15mm), number of retrieved oocytes and number of transfered embryos was comparable between infertile patients with different FSH receptor geno- type. However, patients who carry TT (thr-thr) and NN (asn-asn) genotype need fewer days of ovarian stimulation to oocyte retrieval. (TT: 9,07 days, p1⁄40,028; NN: 9,08 days, p1⁄40,037). A trend of better pregnancy rate in the AA genotype carrying patients was found, although not statistically signifi- cant. The dose of FSH required for a successful stimulation was similar in the two polymorphisms. CONCLUSIONS: FSH receptor genotype is not different between infertile and fertile patients. TT and NN genotype acchieve a significantly higher ovarian response. There is a not significant trend for better pregnancy rate in AA subgroup patients. Our results seem to support some role of FSH re- ceptor polymorphism in infertile patients: the clinical relevance of this factor is however probably low.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
