Up to few years ago, intercellular communication was thought to be regulated exclusively through cell-cell junctions or via exchange of soluble biomolecule messengers. However, a third way is recently strongly emerging: cells also send information by secreting micro- and nanosized extracellular vesicles (EVs), named exosomes, ectosomes and apoptotic bodies. These soft colloidal objects encode information in their content, proteins and nucleic acids, which are protected by a membranous surface tailored for long-distance circulation and cell targeting. EVs participate not only in regulation of physiological processes (such as stem cell maintenance or immune surveillance) but also in the pathology underlying several diseases , which fosters a wealth of therapeutic opportunities . However, while bioanalytical techniques to study EV biology in-vitro and in-vivo are becoming available, knowledge on their properties at the mesoscale is very scarce. As a consequence, (nano)medical applications inherently based on surface- and nanoscience – for example as nanocargos for drugs and biologicals, therapeutic targets or means for liquid biopsy – are languishing . Our contribution will present one of the first stories of integration of molecular biology with colloid chemistry in EV research. We combined the multiscale description of nanoparticle-lipid membrane interaction  with colloidal nanoplasmonics and the fact that nanoparticle aggregation at lipid membranes is modulated by the presence of a protein corona. This allowed us to realize a cost- effective and fast colorimetric assay for assessing by eye purity and titrate exosomes (also providing a peculiar example of nanoparticle−protein corona exploitation) . This set the bases for implementing a surface plasmon resonance (SPR) biosensor to sort exosomes from Multiple Myeloma patients  and to assess the impact of the residual matrix from different separation techniques on the biological activity of exosome preparations .
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