Mesenchymal stromal cells (MSC) are nonhematopoietic, multipotent progenitor cells that are considered one of the most promising product for cellular therapy applications. Initially expanded from bone marrow, MSC can be culture-expanded from different other sources (i.e., umbilical cord blood, synovial membrane, dental pulp, placenta). Recently, adipose tissue (AT) has been regarded as a more suitable and easily accessible source of MSC. The designation of MSC requires that in vitro-expanded cells be plastic-adherent, express surface CD73, CD90 and CD105 but not the hematopoietic markers CD14, CD34, CD45 and the ability to differentiate under specific in vitro culture conditions into osteoblastic, adipocytic and chondroblastic lineages. We will show our characterization of AT-derived MSC, comparing cell lines derived from subcutaneous (lipoaspirate, L) or visceral (omental, O) white fat. The results show significant differences between cell lines in terms of growth characteristics, differentiation properties, immunosuppression capacity, and angiogenic potential of their conditioned medium. We are convinced that the identification of the peculiarities of MSC isolated from different tissues will lead to a more targeted use in cell therapy.
Fat-derived mesenchymal stromal cells: different sources, different properties
DELL'ERA, Patrizia
2012-01-01
Abstract
Mesenchymal stromal cells (MSC) are nonhematopoietic, multipotent progenitor cells that are considered one of the most promising product for cellular therapy applications. Initially expanded from bone marrow, MSC can be culture-expanded from different other sources (i.e., umbilical cord blood, synovial membrane, dental pulp, placenta). Recently, adipose tissue (AT) has been regarded as a more suitable and easily accessible source of MSC. The designation of MSC requires that in vitro-expanded cells be plastic-adherent, express surface CD73, CD90 and CD105 but not the hematopoietic markers CD14, CD34, CD45 and the ability to differentiate under specific in vitro culture conditions into osteoblastic, adipocytic and chondroblastic lineages. We will show our characterization of AT-derived MSC, comparing cell lines derived from subcutaneous (lipoaspirate, L) or visceral (omental, O) white fat. The results show significant differences between cell lines in terms of growth characteristics, differentiation properties, immunosuppression capacity, and angiogenic potential of their conditioned medium. We are convinced that the identification of the peculiarities of MSC isolated from different tissues will lead to a more targeted use in cell therapy.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
